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THALLINGER LAB
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MICROARRAY FACILITY

MICROARRAY

2K microRNA Chip - covering all human, mouse, and rat microRNAs of miRBase v.16
30K Human Oligo-Chip - covering human protein-coding genes
If you are interested in genome-wide expression profiling studies for protein-coding or/and non-coding genes,
please contact marcel.scheideler@tugraz.at.


DATABASE for Microarray Data

MARS Database - Microarray Analysis and Retrieval System


SOFTWARE for Gene Expression Analysis

GenePix - Bioanalytical software for microarray image analysis
ArrayNorm - Software for normalisation and statistical analysis of microarray data
Genesis - Software for visualizing and analyzing gene expression experiments
ClueGO - Software for mapping expression signatures onto biological pathways


HIGH THROUGHPUT MICROARRAY SPOTTER

Biorobotics Microgrid II
120 slides, 24 microplates, humidity control, cooled plate cabinet
Printhead with 48 SMP3 TeleChem pins for >30.000 elements/slide

MICROARRAY SCANNER

Axon instruments GenePix 4000B
Dual laser excitation at 532nm and 635 nm
5 µm resolution

2 HYBRIDIZATION STATIONS

TECAN HS 400 Hybridization Stations
Fully automated hybridization of microarrays, 4 slides per run
Incubations may be performed in the range 4–85°C
Possibility of 6 on-board reagents (4 of them pre-heated)

BIOANALYZER

Agilent 2100 Bioanalyzer
Utilizing Caliper's LabChip® approach for RNA, DNA, protein and cell application.

REAL TIME PCR

ABI PRISM® 7000 Sequence Detection System
Peltier-based, 96-well block thermal cycling system
Multicolor detection provides flexibility for multiplex quantitation assays

HIGH THROUGHPUT THERMAL CYCLER

MJ Research PTC 225 Tetrad
For high-throughput PCR amplifications 4 blocks with 96 well

SPECTROPHOTOMETER

NanoDrop® ND-1000
for measuring absorbance of DNA, RNA, dyes, proteins and microbial cell culture OD
1 µl sample volume, 10 second measurement, 2-3700 ng/ul dsDNA dynamic range