Scientific Volume Imaging

Experts in microscopic imaging

Scientific Volume Imaging develops high quality restoration (deconvolution) and visualization software for microscopists all over the world.

One single Huygens software license for deconvolution and visualisation software can be run simultaneously as often as needed from all computers in a network via a simple X-server tool (http://www.svi.nl/solutions/display.php). Running on five platforms, 64 bit since 1999 and with good memory management Huygens can handle the largest datasets with consummate ease for Widefield, Brightfield, Confocal, Multi-photon, Spinning-disc and 4Pi microscopes. With its native 2D,3D and 4D file reading in ICS, ICS2, all types of Tiffs, Leica Tiffs, Zeiss LSM5, Metamorph STK, MRC, Olympus FluoView, BioVision and DeltaVision Huygens gives a smooth and complete follow up on the acquisition software delivered with your microscope.

Some key features:

Runs on Windows, Linux, Mac, Irix SGI and AIX IBM.
Three basic packages (Essential, Professional and Scripting) ranging from a easy Wizard approach until fully automated deconvolution of multiple imagestacks can all share the same options.
Supports multiprocessing, 64 bit.
Automatic Z-drift, bleaching correction and correction for spherical abberation.
Automatic measurement of various depth dependent PSF's based either on beads or the parameter information.
Many visualisation tools right under your mouse button: Surface & Volume rendering, Twin slicer, MIP renderer.
Analysis tools for all kinds of measurement and Colocalisation
Free support and updates for a full year.

SVI's dedicated team of programmers stays in close contact with the international scientific microscopic community to continuously improve and expand Huygens. Therefore SVI has set up the SVI-Wiki. This is a rapidly expanding public knowledge resource on 3D microscopy and deconvolution. Based on the WikiWikiWeb principle, it is open to contributions from every visitor. In addition it serves as a support medium for SVI customers and relations to discuss different aspects of SVI's Huygens Software.The SVI-wiki can be found in http://support.svi.nl/wiki/

Deconvolution & Visualization Software

Huygens® Professional

Discover the truth behind your image!

With Huygens® Professional you can restore images without being a specialist on image restoration and optical theory. For instance, intelligent tools make it possible for you to obtain the instrumental Point Spread Function of your microscope from an easily recorded latex bead image. This enables you to make sure that your microscope is indeed functioning optimally and ensures optimal restoration results.

Huygens® Essential

Deconvolution a few mouse clicks away!

Huygens® Essential is an image processing software package tailored for deconvolution of microscopic images. Its wizard driven user interface guides you through the process of deconvolving microscopic images. Huygens Essential is also equipped with a special tool that enables you to go through the volume image slice by slice and a tool to extract an PSF from images. Essential can also be extended with the two visualization options Surface Renderer and Colocalization Tool.

Huygens® Scripting

Process your images with automated scripts!

Once you know how to deal with a particular kind of dataset and are sure of the parameters, you can run a couple or more of the datasets through Huygens® Scripting. This is called batch processing. The Huygens Scripting enables you to run scripts written in Tcl, using the extensive set of Huygens image processing commands. With Huygens Scripting you may construct programs that launch multiple parallel image processing jobs to make full usage of a multiple cpu system.

Free Visualization Software

FreeSFP

FREE Volume Rendering with one push on a button!

FreeSFP is a free of charge volume renderer that enables you to explore 3D digital images from a selectable viewpoint. It is especially suitable for single or multi-channel images from microscopes or MRI. The computational work is done by the Simulated Fluorescence Process (SFP) algorithm that takes the volume image as a distribution of fluorescent material, simulating what happens if the material is excited and how the subsequently emitted light travels to the observer.

Usable images

Macrophage fluorescently stained for tubulin (yellow/green), actin (red) and the nucleus (DAPI, blue) recorded with a widefield microscope. Visualized by FluVR's spectral fluorescence volume renderer.

Left part: original data.
Right part: as deconvolved with Huygens Professional.

Recorded by Dr. James Evans.

Macrophage fluorescently stained for tubulin (yellow/green), actin (red) and the nucleus (DAPI, blue) recorded with a widefield microscope as deconvolved with Huygens Professional and visualized by FluVR's spectral fluorescence volume renderer.

Recorded by Dr. James Evans.

This specimen is an isolated Rat Hepatocyte couplet as deconvolved with Huygens Professional and visualized with FluVR's SFP Rendering engine.

Recorded by Lab of Prof. Lukas Landmann.

A metaphase human cell stained for DNA (red), centromeres (blue) and the anaphase promoting complex/cyclosome (green). Visualized by FluVR's spectral fluorescence volume renderer.

Upper part: original data.
Lower part: deconvolved with Huygens Professional.

Recorded by Dr. Claire Acquaviva, Dr. Pines Lab.

Tube-like intranuclear lamin structure traversing the nucleus of a living cell as visualized with green fluorescent protein (GFP). Original data deconvolved with the Huygens System and volume rendered with the GSFP renderer of FluVR.

Data Courtesy: Dr. J.Broers

Nucleus of a human epithelium cell stained with an antibody against splicing factor.

Top part: image as restored by Huygens Professional.
Bottom part: original image.

Both top and bottom images were visualized using the Generalized Simulated Fluorescence Process (GSFP) volume rendering method.

Recorded by Dr. Marjolein A. Grande

Close ups of Macrophage as deconvolved by Huygens Professional, recorded by Dr. James Evans