Supplementary
material Gene Expression Profiling of Human Mesenchymal Stem Cells derived from Bone Marrow during Expansion and Osteoblast Differentiation |
Birgit Kulterer1, Gerald Friedl2, Anita Jandrositz3,
Fatima Sanchez-Cabo1, Andreas Prokesch1, Christine Paar1,
Marcel Scheideler1, Reinhard Windhager2, Karl-Heinz Preisegger3, Zlatko Trajanoski1 |
Addresses: 1Institute for Genomics and Bioinformatics and Christian-Doppler Laboratory for Genomics and Bioinformatics, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria; 2Department of Orthopaedics, Medical University of Graz; 3Eccocell Biotechnology Inc., Graz, Austria |
Correspondence: Zlatko Trajanoski. Email: Zlatko.Trajanoski@tugraz.at URL:http://genome.tugraz.at |
Additional datafiles and analysis results |
1. | FACS Analysis: Surface profiles in different passages [pdf] | |
2. | Expression of stem cell specific marker genes evaluated by Realtime RT-PCR [pdf] | |
3. | Array Design: Information about spotted oligos [xls] | |
4. | Standard Operating Procedure (SOP) for AMINOALLYL LABELING OF RNA FOR HUMAN OLIGO CHIPS [pdf] | |
5. | Standard Operating Procedure (SOP) for HUMAN OLIGO CHIP PROBE HYBRIDIZATION [pdf] | |
6. | Differentially expressed ESTs (>2-foldchange) when comparing p2/p5 and p5/p10 for following moderated t-test [xls] | |
7. | Validation of Microarray data by independent Realtime RT- PCR of long-term cultivated hMSC [pdf] | |
8. | Gene expression of osteoblast specific genes determined by Real-time RT-PCR to prove the osteogenic phenotype [pdf] | |
9. | 1108 selected ESTs from microarray analysis [xls] | |
10. | k-means clustering of 1108 selected ESTs [pdf] | |
11. | Figure of merit analysis for validation of the k-value [pdf] | |
12. | Hierarchical clustering of 1108 selected ESTs [png] | |
13. | Validation of Microarray data by independent Realtime RT- PCR of hMSC during Osteogenesis [pdf] | |
14. | Distribution of gene ontology (GO) terms in each cluster [png] | |
15. | Expression matrix of differentially expressed ESTs (>2-foldchange) [png] | |
16. | 659 selected ESTs from microarray analysis [xls] | |
17. | Validation of microarray data by real-time RT-PCR data: hOB compared with hMSC during osteogenesis[pdf] | |
18. | Comparison of human osteoblasts with in vitro differentiated hMSC [pdf] |