Supplementary material

Gene Expression Profiling of Human Mesenchymal Stem Cells derived from Bone Marrow during Expansion and Osteoblast Differentiation
Birgit Kulterer1, Gerald Friedl2, Anita Jandrositz3, Fatima Sanchez-Cabo1, Andreas Prokesch1, Christine Paar1, Marcel Scheideler1,
Reinhard Windhager2, Karl-Heinz Preisegger3, Zlatko Trajanoski1
Addresses: 1Institute for Genomics and Bioinformatics and Christian-Doppler Laboratory for Genomics and Bioinformatics, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria; 2Department of Orthopaedics, Medical University of Graz; 3Eccocell Biotechnology Inc., Graz, Austria
Correspondence: Zlatko Trajanoski. Email: URL:

Additional datafiles and analysis results

  1. FACS Analysis: Surface profiles in different passages [pdf]
  2. Expression of stem cell specific marker genes evaluated by Realtime RT-PCR [pdf]
 3. Array Design: Information about spotted oligos [xls]
 4. Standard Operating Procedure (SOP) for AMINOALLYL LABELING OF RNA FOR HUMAN OLIGO CHIPS [pdf]
 5. Standard Operating Procedure (SOP) for HUMAN OLIGO CHIP PROBE HYBRIDIZATION [pdf]
 6. Differentially expressed ESTs (>2-foldchange) when comparing p2/p5 and p5/p10 for following moderated t-test [xls]
  7. Validation of Microarray data by independent Realtime RT- PCR of long-term cultivated hMSC [pdf]
 8. Gene expression of osteoblast specific genes determined by Real-time RT-PCR to prove the osteogenic phenotype [pdf]
 9. 1108 selected ESTs from microarray analysis [xls]
 10. k-means clustering of 1108 selected ESTs [pdf]
 11. Figure of merit analysis for validation of the k-value [pdf]
 12. Hierarchical clustering of 1108 selected ESTs [png]
 13. Validation of Microarray data by independent Realtime RT- PCR of hMSC during Osteogenesis [pdf]
 14. Distribution of gene ontology (GO) terms in each cluster [png]
 15. Expression matrix of differentially expressed ESTs (>2-foldchange) [png]
 16. 659 selected ESTs from microarray analysis [xls]
 17. Validation of microarray data by real-time RT-PCR data: hOB compared with hMSC during osteogenesis[pdf]
 18. Comparison of human osteoblasts with in vitro differentiated hMSC [pdf]